This article is not medical or healthcare advice. Before starting any health related regimen, seek the advice of your Primary Care Physician or an M.D.
Peroxynitrite is formed when super oxide and nitric oxide combine. It is a potent free radical, and based on dozens of clients, i have observed a very specific set of genetic patterns that provide fertile ground for the over production of Peroxynitrite. Peroxynitrite is at the heart of some of the earlier studies on ME CFS, but because bio markers are limited to detect it - its been an elusive topic. However, Vibrant America's new oxidative stress panel - gives us 16 oxidative stress biomarkers - so we can which radical - hydrogen peroxide, hydroxyl radical , super oxide, peroxynitratite, or carbonate radicals are causing the most damage. This usually occurs when there is a combination of things present, listed in order of importance:
Pattern 1: NOS Uncoupling
Upregulation of NOS2
Downregulation of NOS3
Compromise in BH4 production and or recycling
Compromise in Sirt 1 and or Sirt 3
Pattern 2: Compromise in Anti Oxidant Systems that neutralize Peroxynitrite and or Super Oxide
Compromise in GPX1 (glutathione peroxidase 1)
Pattern 3: Compromise in Anti Oxidant Systems that neutralize Super Oxide
Compromise in SOD2 and or F12
Compromise in NQO1 and or Pon1
Compromise in B12 transport and or absorption
Pattern 4: Compromise in Master Controller Anti Oxidant Genes
Downregulated NrF2
Upregulated Keap 1
Upregulated Bach1
A new research finding indicates that high levels of peroxynitrite inhibits SOD2. Further stimulation of SOD2 by supplements in the presence of high levels of perxoynitrite results in SOD2 being suppressed by 97% !
It appears the first order of business in anti oxidant therapy is to assess and take of excess peroxynitrite - and to do this - having a map of which pathways/genes are compromised is invaluable.
Peroxynitritite reacts with the carbonate radical and can cause nitrative stress, DNA/RNA damage, and protein oxidation, in addition to rapdily oxidizing BH4 to BH2.